Year: 2023
Type: review
Abstract: Kidney organoids possess the potential to revolutionize the treatment of renal diseases. However, their growth and maturation are impeded by insufficient growth of blood vessels. Through a PubMed search, we have identified 34 studies that attempted to address this challenge. Researchers are exploring various approaches including animal transplantation, organ-on-chips, and extracellular matrices (ECMs). The most prevalent method to promote the maturation and vascularization of organoids involves transplanting them into animals for in vivo culture, creating an optimal environment for organoid growth and the development of a chimeric vessel network between the host and organoids. Organ-on-chip technology permits the in vitro culture of organoids, enabling researchers to manipulate the microenvironment and investigate the key factors that influence organoid development. Lastly, ECMs have been discovered to aid the formation of blood vessels during organoid differentiation. ECMs from animal tissue have been particularly successful, although the underlying mechanisms require further research. Future research building upon these recent studies may enable the generation of functional kidney tissues for replacement therapies.
Source: Biology
Authors: Ran Konoe, Ryuji Morizane
Institutions: Harvard University, Massachusetts General Hospital
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,447
Awards: G1011863915, G1665875804, Ex Vivo Generation of Functional Kidney Tissues for Transplantation, G356500296, G4301765064, Recreating Kidney Organogenesis in vitro with Human Pluripotent Stem Cells, Vascularized kidney organoids on chip for efficacy and toxicity testing of somatic genome editing, Development of a fluidic chip model of PKD to elucidate cystogenic signals using kidney organoids, G797069571, Vascularized kidney organoids on chip for efficacy and toxicity testing of somatic genome editing, G8237161393
OpenAlex ID: W4361005101