Supplementary Figures and Tables from SDHD Promoter Mutations Ablate GABP Transcription Factor Binding in Melanoma
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figure 1. Confirmation of the recurrent C524, C541 and C544 SDHD promoter mutations in melanoma cell lines via Sanger sequencing. Supplementary Figure 2. Expression of SDHD in melanomas harboring promoter mutations compared to SDHD wild-type samples across TCGA samples. Supplementary Figure 3. Luciferase reporter assays for the C541T and C544T SDHD hotspot promoter mutations in multiple melanoma cell lines. Supplementary Figure 4. Prediction of the consequences of the C541T and C544T SDHD promoter mutations on transcription factor binding. Supplementary Figure 5. mRNA expression correlation between SDHD and multiple ETS transcription factors in TCGA SKCM samples harboring the SDHD C523T promoter mutation. Supplementary Figure 6. Band-shift analysis of recombinant human ELF1 binding to wild-type and C523T, C524T, C541T, and C544T SDHD promoter sequences. Supplementary Figure 7. Effect of siRNA-mediated knockdown of ELF1 on SDHD expression in melanoma cells. Supplementary Figure 8. Effect of siRNA-mediated knockdown of PRDM1 or IRF4 on SDHD expression and SDHD promoter activity in melanoma cells. Supplementary Table 1. SDHD promoter mutations identified in melanoma tumors datasets (TCGA, Broad and Yale) and melanoma cell lines. Supplementary Table 2. MotifBreakR results predicting the effects of recurrent SDHD promoter mutations (C523T, C524T, C541T, C544T, and C548T) on transcription factor binding sites. Supplementary Table 3. Oligonucleotide design for quantitative mass spectrometry.
Authors: Tongwu Zhang, Mai Xu, Matthew M. Makowski, Christine Lee, Michael Kovacs, Jun Fang, Esther Willems, Jeffrey M. Trent, Nicholas K. Hayward, Michiel Vermeulen, Kevin M. Brown
Language: English
Topic: Melanoma and MAPK Pathways
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361236413