Supplementary Figures from Differential Regulation of the Melanoma Proteome by eIF4A1 and eIF4E
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figures - Figure S1. Representative western blots demonstrating knockdown and overexpression of elF4A1 and elF4# in MSTC and A375. Figure S2. Western blots validating factor-specific and commonly regulated proteins using primary and secondary siRNAs. Figure S3. Knockdown of eIF4A1 or eIF4E for 48h does not increase apoptosis. Figure S4. Survival data for individual cell cycle proteins. Figure S5. Inhibition of the electron transport chain reduces A375 invasion. Figure S6. Cell Cycle and TCA/ETC pathways are less enriched at the mRNA level than at the protein level. Figure S7. mRNA determinants of direct regulation by eIF4A1 and eIF4E are not shared by indirect targets. Figure S8. Increased density of CERT motifs in 5'UTR and 3'UTR of transcripts that are positively regulated by eIF4E. Figure S9. Ribosomal subunit mRNA, not protein, levels are increased with eIF4E depletion. Figure S10. Reduced general translation upon eIF4A1 and eIF4E depletion is rescued by miRNA-target interactions.
Authors: Cailin E. Joyce, Adrienne G. Yanez, Akihiro Mori, Akinori Yoda, Johanna S. Carroll, Carl D. Novina
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361256899