Year: 2023
Type: review
Abstract: High molecular weight (MW), highly repetitive protein polymers are attractive candidates to replace petroleum-derived materials as these protein-based materials (PBMs) are renewable, biodegradable, and have outstanding mechanical properties. However, their high MW and highly repetitive sequence features make them difficult to synthesize in fast-growing microbial cells in sufficient amounts for real applications. To overcome this challenge, various methods were developed to synthesize repetitive PBMs. Here, we review recent strategies in the construction of repetitive genes, expression of repetitive proteins from circular mRNAs, and synthesis of repetitive proteins by ligation and protein polymerization. We discuss the advantages and limitations of each method and highlight future directions that will lead to scalable production of highly repetitive PBMs for a wide range of applications.
DOI: 10.3390/ijms24076416
Institution: Washington University in St. Louis
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,780
Awards: G1286565498, G1951469277, G2116509936, G302854842, Structure-Property Relationships of Protein-based Block Copolymer Nanocomposites, Designing and Understanding High-performance Titin Polymers Using Synthetic Biology, G6509530478, G7118703037, G7118795109, Collaborative Research: GCR: Accelerated Discovery of Synthetic Biological Materials, Targeted delivery of a proangiogenic and promyogenic protein for regeneration of diabetic ischemic limbs, G936628054
OpenAlex ID: W4361268242