Work

Prime editing: advances and therapeutic applications

Year: 2023

Type: review

Abstract: Clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR-Cas)-mediated genome editing has revolutionized biomedical research and will likely change the therapeutic and diagnostic landscape. However, CRISPR-Cas9, which edits DNA by activating DNA double-strand break (DSB) repair pathways, is not always sufficient for gene therapy applications where precise mutation repair is required. Prime editing, the latest revolution in genome-editing technologies, can achieve any possible base substitution, insertion, or deletion without the requirement for DSBs. However, prime editing is still in its infancy, and further development is needed to improve editing efficiency and delivery strategies for therapeutic applications. We summarize latest developments in the optimization of prime editor (PE) variants with improved editing efficiency and precision. Moreover, we highlight some potential therapeutic applications.

Source: Trends in biotechnology

DOI: 10.1016/j.tibtech.2023.03.004

Authors: Zhihan Zhao, Peng Shang, Prarthana Mohanraju, Niels Geijsen

Institutions: Leiden University Medical Center, Novo Nordisk Foundation Center for Stem Cell Medicine

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $6,830

Funders: China Scholarship Council, Novo Nordisk Fonden