Supplementary Video 1 from A Preclinical Assay for Chemosensitivity in Multiple Myeloma
Year: 2023
Type: supplementary-materials
Abstract: MOV file - 1415K, Patient-derived MM cells (patient 14) were seeded in single (top left) and co-culture with patient-derived stroma cells (top right). They were tested for chemosensitivity to melphalan (continuous exposure for 24h, at highest concentration of 25uM, lowest concentration of 5uM). Digital image analysis algorithm detects live cells and pseudo-colors them as green. MM cells in single culture are significantly more sensitive than in co-culture, a phenomenon commonly referred to as environment-mediated drug resistance (EMDR). At 1nM, bortezomib shows no toxicity to these cells after a 24h continuous exposure (bottom left), but in combination with melphalan, it cancels EMDR (bottom right).
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361810074