Supplementary Figure 2 from SIRT1 Pathway Dysregulation in the Smoke-Exposed Airway Epithelium and Lung Tumor Tissue
Year: 2023
Type: supplementary-materials
Abstract: PDF file,79K, Pathway activation predictions for each control dataset. A. ATM pathway activation is increased in H1299 and transiently decreased and then restored in H460 cells exposed to 2 Gy of ionizing radiation (IR). RNA from the cells was sampled pre-exposure (n=12) and at various time points post-exposure (n=30) and hybridized to Affymetrix Gene 1.0 ST microarray (GSE20549). B. GPX1 pathway activation is decreased in response to a selenium deficient diet. Rats were fed selenium deficient diets (n=3) or supplemented with sodium selenite up to 5ug/g for 28 days (n=15). Rat livers were harvested and RNA was isolated and hybridized to Affymetrix Rat Genome 230 2.0 microarrays (GSE23895). C. IKBKB pathway activation is increased after retrovirus infection with the IKBKB. H929 and SACHI cells lines were infected with retroviruses containing MAP3K14 (n=6), IKBKB (n=6) compared with controls were no infection was conducted (n=6). RNA was isolated and hybridized to Affymetrix HGU133 Plus 2.0 microarrays (GSE18047). D. SIRT1 pathway activation is increased in A549 cells treated with resveratrol. A549 cells treated with an ethanol control (n=1) or 25 microM of resveratrol (n=3) for 48h and RNA was hybridized to Affymetrix HGU133 Plus 2.0 microarrays (GSE9008). E. SIRT1 pathway activity is decreased in the SIRT1 knockdown compared to the control. MCF-7 cells were treated with two retroviral constructs, a shRNA to knockdown SIRT1 (n=3), and a shRNA directed against Luciferase (n=3) and RNA was hybridized to Affymetrix HGU133A 2.0 microarrays (GSE13459). F. SIRT1 pathway activation was increased in cells treated with tobacco smoke condensate (TSC). A549 cells or Calu-6 cells were cultured with and without TSC and hybridized to Affymetrix HGU133 Plus 2.0 microarrays (GSE13309). For each cells type, there were 3 controls and 9 samples treated with TSC.
Language: English
Subfield: Geriatrics and Gerontology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361840904