Supplementary Figure 2 from Estrogen Receptor Alpha Mediates Progestin-Induced Mammary Tumor Growth by Interacting with Progesterone Receptors at the Cyclin D1/MYC Promoters
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 138K, (A) Left. Purified C4-HD cells were cultured in chamber slides. After attachment, the medium was replaced and the cells starved in 1% chFCS for 24 h. Cells were untreated or treated with MPA (10 nM) and/or ICI (1 �M) during 6 h in the presence of BrdU and processed for immunofluorescence and confocal microscopy with specific antibodies: anti-BrdU (green) and cytokeratin (CK, red). Right. Quantification of BrdU+/CK+ cells (mean � SEM) after treatment. (B) Left. 3H-thymidine uptake assays: C4-HD cells were transfected with siRNAs for mouse ER� or a control siRNA, starved, treated for 48 h with MPA and the 3H-thymidine uptake index calculated as described previously. A representative experiment (mean � SEM) of three is shown. Right. Western blots: Protein extracts of C4-HD cells transfected for 48 h with siRNAs for mouse ER� as described previously were used to evaluate the expression of ER� (MC-20) in transfected cells. Erk1/2 was used as a loading control. Cell extracts from C4-HD cells treated with ICI for 24 hs were used as a control. (C) C4-HD cells were starved and then treated with ICI during 6, 12 and 24 h. ER� (MC-20) and PR (C-19) expression was analyzed by Western blot. �-actin (I-19, Santa Cruz) was used as a loading control.
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361845504