Supplementary Data from Comprehensive Mutation and Copy Number Profiling in Archived Circulating Breast Cancer Tumor Cells Documents Heterogeneous Resistance Mechanisms
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figure S1. Clinical timelines. Clinical timelines and treatment from first diagnosis until enrollment into MI-CTC-ONCOSEQ for the 11 metastatic breast cancer patients; Supplementary Figure S2. Sanger sequencing of WGA CTC DNA and Western blot confirming the presence of A569S mutation; Supplementary Figure S3. MCF-7 (ESR1 A569S) are not estrogen independent over 5 days in hormone-free conditions; Supplementary Figure S4. The tamoxifen metabolites 4-hydroxytamoxifen and endoxifen have no increased agonistic effect in MCF-7 cells over-expressing ER-A569S; Supplementary Figure S5. Inhibition of estradiol-stimulated MCF-7 parental or A569S- overexpressing cells. Supplementary Table S1. Oncomine Comprehensive Panel (OCP) target genes by presence of the MseI restriction site; Table S2. NGS of BT-474 cells spiked into blood and purified identifies expectedTP53 mutation; Supplementary Table S3. Results of CTC purification by DEPArray; Supplementary Table S4: Mean CTC Next Generation Sequencing parameters; Supplementary Table S5. NGS-identified prioritized mutations in individual and pooled CTC samples; Supplementary Table S6. 2 x 2 contingency tables of concordance for alterations in CTC vs tissue; Supplementary Table S7. Patient #2 analysis of primary, clinical metastatic tissue, research biopsy, pt-DNA by ddPCR; Supplementary Table S8. Primer/probe sequences used in Sanger sequencing and ddPCR.
Authors: Costanza Paoletti, Andi K. Cani, Jose M. Larios, Daniel H. Hovelson, Kimberly Aung, Elizabeth P. Darga, Emily M. Cannell, Paul J. Baratta, Chia-Jen Liu, David Chu, Maryam Yazdani, Allen R. Blevins, Valeria Sero, Nahomi Tokudome, Dafydd G. Thomas, Christina Gersch, Anne F. Schott, Yi-Mi Wu, Robert Lonigro, Dan R. Robinson, Arul M. Chinnaiyan, Farideh Z. Bischoff, Michael D. Johnson, Ben H. Park, Daniel F. Hayes, James M. Rae, Scott A. Tomlins
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361847265