Supplemental Figures S1-S12 from Androgen Receptor Is the Key Transcriptional Mediator of the Tumor Suppressor SPOP in Prostate Cancer
Year: 2023
Type: supplementary-materials
Abstract: Reciprocal gene set enrichment analysis reveals that SPOP F102C, F133V, and F133L mutants have similar effects on the PC transcriptome (S1); AR Activity Score of PC cells expressing wt or mutant SPOPs (S2); Effects of wt-SPOP and PC-associated SPOP mutants on mRNA expression of AR target genes in Abl PC cells (S3); The transcriptomic response induced by mt-SPOPs in PC cells enriches strongly for target genes of AR antagonists (S4); The impact of SPOP on AR protein levels in Abl cells is post-translational (S5); Wt-SPOP, but not its PC-associated mutants, suppresses AR protein levels in androgen-dependent PC cells (S6); The interaction between SPOP-wt and AR can occur even in the absence of SRC-3 protein (S7); Interaction and binding of SPOP-wt to AR is mediated by an SBC motif (a.a.646-a.a.651) located in the Hinge Region of the AR protein (S8); Endogenous SPOP interacts with and regulates the expression levels of the AR protein in PC cells (S9); The impact of SPOP on AR protein levels in 22Rv1 cells is post-translational (S10); Growth promoting effect of PC-associated SPOP mutants in the context of AR axis inhibition (S11); SPOP gene signature scores in human PC specimens correlate strongly with androgen receptor activity (S12).
Authors: Chuandong Geng, Kimal Rajapakshe, Shrijal S. Shah, John Shou, Vijay Kumar Eedunuri, Christopher Foley, Warren Fiskus, Mahitha Rajendran, Sue Anne Chew, Martin Zimmermann, Richard Bond, Bin He, Cristian Coarfa, Nicholas Mitsiades
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361848622