Supplemental Figures 1-20 from A Quantitative System for Studying Metastasis Using Transparent Zebrafish
Year: 2023
Type: supplementary-materials
Abstract: Supplemental Figures 1-20. Supplemental Figure 1: Metastatic behavior of primary melanoma transplants. Supplemental Figure 2: Demonstration of cDNA overexpression in the ZMEL1 line. Supplemental Figure 3: Demonstration of CRISPR/Cas9-mediated mutation in the ZMEL1 line. Supplemental Figure 4: SURVEYOR assay to assess mutation rates in ZMEL1 cells nucleofected with CRISPR/Cas9 constructs. Supplemental Figure 5: Gene Set Enrichment Analysis (GSEA) for the ZMEL1 transcriptional signature compared to the NCI60 human cancer cell lines (dataset NCI60GSE5846). Supplemental Figure 6: Heatmap plot of the ZMEL1 transcriptional signature compared to the NCI60 human cancer cell lines (dataset NCI60GSE5846). Supplemental Figure 7: Gene Set Enrichment Analysis (GSEA) for the ZMEL1 compared to transgenic mitf-BRAF;p53 tumors. Supplemental Figure 8: Heatmap plot of the ZMEL1 transcriptional signature compared to transgenic mitf-BRAF;p53 tumors. Supplemental Figure 9: Ingenuity Pathway Analysis of the ZMEL1 expression signature showing canonical pathways. Supplemental Figure 10: Ingenuity Pathway Analysis of the ZMEL1 expression signature showing core enriched networks. Supplemental Figure 11: Heatmap of metastatic spread over 14 days when cells are transplanted dorsally. Supplemental Figure 12: Histologic assessment of fish transplanted with ZMEL1-GFP cells Supplemental Figure 13: Histology of the kidney marrow from a stable transgenic mitfa-BRAFV600E;p53-/- melanoma bearing fish. Supplemental Figure 14: Derivation of the µ score for quantifying metastatic burden Supplemental Figure 15: Calculation of the metastasis initiating cell (MIC) frequency Supplemental Figure 16: Schematic overview of bright field image processing. Supplemental Figure 17: Determining pixel intensities in the transformed images. Supplemental Figure 18: Methods for segmentation of GFP images. Supplemental Figure 19: Methods for clustering of close objects. Supplemental Figure 20: Methods for separating merged tumors/metastases.
Authors: Silja Heilmann, Kajan Ratnakumar, Erin M. Langdon, Emily R. Kansler, Isabella S. Kim, Nathaniel R. Campbell, Elizabeth B. Perry, Amy J. McMahon, Charles K. Kaufman, Ellen van Rooijen, William Lee, Christine A. Iacobuzio-Donahue, Richard O. Hynes, Leonard I. Zon, Joao B. Xavier, Richard M. White
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361849573