Supplemental Figure 1 from Endoplasmic Reticulum Stress Protein GRP78 Modulates Lipid Metabolism to Control Drug Sensitivity and Antitumor Immunity in Breast Cancer
Year: 2023
Type: supplementary-materials
Abstract: LCC9 (A) or ZR-75-1 (B) cells were transfected with control siRNA, GRP78 siRNA, or GRP78 cDNA for 24 hours and then plated in an ACEA E-plate in the presence of vehicle or 100 nM 4-OHT; cell index was measured by electrical impedance every 12 hours for 72 hours. n=3, *p<0.05 C. LCC9 tumor sections were stained using GRP78 antibodies or a no antibody control and visualized at 40x. D. PI3K p110 subunit, pAKT (Ser473 and Thr308), and AKT protein levels were measured in LCC9 control, TAM, human targeting GRP78M, and human targeting GRP78M+TAM treated xenograft tumor lysates by Western blot hybridization. Equivalence of protein loading was confirmed by measuring beta-actin.
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361849834