Supplemental Figures 1 - 5, Figure Legends and Methods from Metabolic Signature Identifies Novel Targets for Drug Resistance in Multiple Myeloma
Year: 2023
Type: supplementary-materials
Abstract: Supplemental Figure 1. Hypoxia-induced drug resistance on MM cells. Supplemental Figure 2. HK2, PFKFB3, PFKFB4 and LDHA expression in MM cell lines. Supplemental Figure 3. (A-B) HIF1A expression in MM1S-shHIF1A. (C) HIF2A expression in MM1S-shHIF2A. (D) Stable knockdown of HIF1A restores the effect of melphalan under hypoxic conditions. (E) HIF1A expression in MM1S-shHIF1A. Supplemental Figure 4. (A-B) HK2 and LDHA expression in MM1S-shHK2, MM1S-shLDHA. (C) Relative mRNA levels of HK2 and LDHA in CD138 + cells 3 from normal donors bone marrow, MGUS, Smoldering myeloma and newly diagnosed myeloma (GSE6477). (D) Stable knockdown of LDHA partially restore the effect of melphalan under hypoxic conditions. (E) PDK1 expression in MM1S-PDK1 expressing cells. (F) Stable overexpression of PDK1 induces resistance to bortezomib in MM1S cells. Supplemental Figure 5. LDHA and HIF1A expression in ANBL6-BRshLDHA, MM1S-HIF1A OE and in MM1S-LDHA OE cells.
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361874603