Supplementary Materials and Methods, Supplementary Figures 1 through 9, and Supplementary Tables 1 through 5 and 7 and 8 from PROTOCADHERIN 7 Acts through SET and PP2A to Potentiate MAPK Signaling by EGFR and KRAS during Lung Tumorigenesis
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Methods, References, and Figure Legends, Supplementary Fig. S1-High magnification images of PCDH7 IHC staining in normal human lung and lung adenocarcinomas. Supplementary Fig. S2-NSCLC tissue microarray analysis of PCDH7 immunoexpression. Supplementary Fig. S3-PCDH7 induces cellular transformation of HBEC cells and induces a gene expression program that promotes cell proliferation. Supplementary Fig. S4-All PCDH7 isoforms induce MAPK signaling in HBEC-shp53-KRASG12V cells. Supplementary Fig. S5-PCDH7 synergizes with mutant EGFR to promote tumor formation. Supplementary Fig. S6-CRISPR/Cas9-mediated inhibition of PCDH7 sensitizes NSCLC cells to MEK and ERK inhibitors. Supplementary Fig. S7-PCDH7 isoforms A, C, and D bind SET in EGFR-mutant cells. Supplementary Fig. S8-SET localization studies in HBECs and NSCLC cells and requirement of SET for PCDH7-induced MAPK pathway activation. Supplementary Fig. S9-Treatment of NSCLC cells with a SET inhibitor FTY720 induces cell death. Supplementary Table S1. Expression and survival analysis of PCDH family members in lung adenocarcinoma. Supplementary Table S2. Affymetrix probe IDs analyzed for each PCDH family member. Supplementary Table S3. Summary of clinical and pathologic information of the cohort of NSCLC patient tissue blocks used for TMA generation. Supplementary Table S4. Analysis of tissue microarray correlations. Supplementary Table S5. Correlation between PCDH7 upregulation and alterations in KRAS or EGFR. Supplementary Table S7. List of antibodies utilized in this study. Supplementary Table S8. List of primer sequences utilized in this study.
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361875242