Supplementary Figures & Tables from RORγt+ Innate Lymphoid Cells Promote Lymph Node Metastasis of Breast Cancers
Year: 2023
Type: supplementary-materials
Abstract: Figure S1, Related to Figure 1 FACS and immunofluorescence validation of Lin-CD127+CD90.2+NKP46- gated ILC3 cells Figure S2, Related to Figure 2 ELISA quantification of CXCL13 and CCL21 chemokines levels in conditioned media (CM) obtained from the bone marrow derived MSC cell line (HS-5) and from the breast cancer cell line 4T1.2 cell line is shown. Figure S3, Related to Figure 2 Proliferation assay of ILC3 cells and ELISA quantification of CXCL13 and CCL21 from siRNA transfected MSC cells. Figure S4, Related to Figure 4 CXCR5 expression on MSC cells. Figure S5, Related to Figure 5 Identification of a RORγT+CD127+CD3- ILC3 cells within the tertiary lymphoid structures within breast cancers. Figure S6, Related to Figure 5 Expression of lymphoid chemokine and chemokine receptor genes in breast cancer datasets. Table S1, Related to Figure 5 Table S1: Clinico-pathological characteristics for the METABRIC sample.
Authors: Sheeba Irshad, Fabian Flores-Borja, Katherine Lawler, James Monypenny, Rachel Evans, Victoria Male, Peter Gordon, Anthony Cheung, Patrycja Gazinska, Farzana Noor, Felix Wong, Anita Grigoriadis, Gilbert O. Fruhwirth, Paul R. Barber, Natalie Woodman, Dominic Patel, Manuel Rodriguez-Justo, Julie Owen, Stewart G. Martin, Sarah E. Pinder, Cheryl E. Gillett, Simon P. Poland, Simon Ameer-Beg, Frank McCaughan, Leo M. Carlin, Uzma Hasan, David R. Withers, Peter Lane, Borivoj Vojnovic, Sergio A. Quezada, Paul Ellis, Andrew N.J. Tutt, Tony Ng
Institutions: King's College London, Breast Cancer Now, Centre International de Recherche en Infectiologie, École Normale Supérieure de Lyon, Cancer Research UK Scotland Institute, University of Oxford, CRUK/MRC Oxford Institute for Radiation Oncology, Guy's Hospital, Kings Health Partners, Lyon 1 Université, Centre National de la Recherche Scientifique, Inserm, Hospices Civils de Lyon, Nottingham University Hospitals NHS Trust, Nottingham City Hospital, University of Cambridge, University of Birmingham, University College London, Guy's and St Thomas' NHS Foundation Trust
Language: English
Topic: IL-33, ST2, and ILC Pathways
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361876171