Supplementary Figures from HER2 Overexpression Triggers an IL1α Proinflammatory Circuit to Drive Tumorigenesis and Promote Chemotherapy Resistance
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figures with legends- Supplementary Figures S1 to S14. S1. Shows gene ontology enrichment analysis between MCF10A-HER2 and MCF10A-vector cells. S2 shows western blot analysis of HER2 signaling after IL-1β treatment. S3, S4 and S9 show knockout of IL1A and IL6 using CRISPR/Cas9 system. S5 shows western blot analysis of HER2-signalign pathways in cells treated with IRAK1 and JAK2 inhibitors. S6 shows that activation of NF-κB and STAT3 is not mediated by HER2-immediate downstream molecules. S7 and S10 shows western blot analysis of total HER2, phosphorylated p65, STAT3 and AKT in mammary gland tissues and primary tumors. S8 shows the effects of blockades of HER2-downstream signaling pathways on cell viability. S11 shows representative images of Immunohistochemical staining of HER2, IL-1α and IL6 in primary breast cancer tissues. S12 shows Kaplan-Meier plots of distant metastasis-free survival of patients. S13 shows combination drug effects of IRAK inhibitor (IRAKi) plus paclitaxel (PCL) or cisplatin (CPT). S14 shows tumor volume growth curves for individual mice after last treatment.
Language: English
Topic: HER2/EGFR in Cancer Research
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361876831