Supplementary Figure 5 from Inactivation or Loss of TTP Promotes Invasion in Head and Neck Cancer via Transcript Stabilization and Secretion of MMP9, MMP2, and IL-6
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 4600K, Supplementary Figure S5. TTP regulates the stability of transcripts IL-6, MMP9 and MMP2 via the 3'-UTR. (A) Left three panels: IL-6, MMP9 and MMP2 mRNA levels were quantified via Q-RT-PCR from UM-SCC-81B cells transfected with NT and siTTP. Data are representative of two independent experiments with three replicates in each experiment. Right Panel: Whole-cell lysates were immunoblotted with TTP and actin antibodies. Signal intensity was quantified, normalized to actin, and expressed as percent of NT. (B) Left three panels: UM-SCC-81B cells transfected with NT or siTTP were then co-transfected with a luciferase reporter construct containing the 3'-UTR of IL-6, MMP9 or MMP2 and a Renilla construct for normalization. Experiments were performed in triplicate with three replicates in each experiment. Right panel: Whole cell lysates were immunoblotted with TTP and actin antibodies. Signal intensity was quantified, normalized to actin, and expressed as percent of NT. (*p<0.01)
Language: English
Topic: RNA Research and Splicing
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361882706