Supplementary Figure 4 from The Nedd8-Activating Enzyme Inhibitor MLN4924 Thwarts Microenvironment-Driven NF-κB Activation and Induces Apoptosis in Chronic Lymphocytic Leukemia B Cells
Year: 2023
Type: supplementary-materials
Abstract: MLN4924 leads to downregulation of NFκB activity in a dose-dependent manner. (A) MLN4924 leads to downregulation of CXCR5 in CLL. CLL cells (N=9) were co-cultured with CD40L-expressing stroma for 24 hours, followed by incubation with 1 μM MLN4924 or vehicle control for 24 h. Total RNA was isolated from CD19+ CLL B-cells, reverse-transcribed and subjected to real-time PCR with the indicated probes (in duplicates). Results were normalized to 18S levels. (B-C) CLL cells (N=4) were co-cultured with CD40L-expressing stroma for 24 hours, followed by incubation with 0.25 or 1 μM MLN4924 or vehicle control for another 24 h. Nuclear protein fractions were isolated and p50 and p65 activity was measured by ELISA as per manufacturer's instructions (B). Total RNA was reverse-transcribed (C) and subjected to real-time PCR with the indicated probes (in duplicates). Results were normalized to 18S levels. Measurements were normalized to vehicle control and reported as a fold change. * - p<0.05 and ** - p<0.01 compared to untreated control.
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361883005