Supplementary Figure 4 from Prostate Cancer Progression Correlates with Increased Humoral Immune Response to a Human Endogenous Retrovirus GAG Protein
Year: 2023
Type: supplementary-materials
Abstract: - PDF file 418K, Characterization of TI-35 mAb by subcellular colocalization of staining with GAG-HERV-K expression and by transfection. A-B, Confocal images of HeLa cells transfected with GAG-HERV-K_GFP (A) or GFP only (B) and stained with TI-35. Blue represents DAPI nuclear staining, green represents GFP, and purple represents TI-35 staining. Colocalization is observed in the merged data as white, in areas indicated by arrows. Axes in the corner of each quadrant show size/magnification information. C-D, TI-35 immunostaining with formalin-fixed Sf9 insect cells infected with GAG-HERV-K-recombinant baculovirus (C) or control baculovirus (D). Original magnification (objective) was 4x, and insets show zoomed area at 20x
Authors: Bernardo Sgarbi Reis, Achim A. Jungbluth, Denise Frosina, Megan Holz, Erika Ritter, Eiichi Nakayama, Toshiaki Ishida, Yuichi Obata, Brett Carver, Howard Scher, Peter T. Scardino, Susan Slovin, Sumit K. Subudhi, Victor E. Reuter, Caroline Savage, James P. Allison, Jonathan Melamed, Elke Jäger, Gerd Ritter, Lloyd J. Old, Sacha Gnjatic
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361883356