Supplementary Figures 1 - 7 from Multiplexed Quantitative Analysis of CD3, CD8, and CD20 Predicts Response to Neoadjuvant Chemotherapy in Breast Cancer
Year: 2023
Type: supplementary-materials
Abstract: Supplemental Figure 1. Schematic for multiplexed immunofluorescence staining for tumor infiltrating lymphocytes. Supplemental Figure 2. Comparison between AQUA scores within the total compartment and AQUA scores calculated within the stromal compartment and tumor mask. Supplemental Figure 3. Reproducibility of quantitative immunofluoresence assay on serial sections of an invasive breast tissue microarray. AQUA scores for (A) stromal CD3 expression, (B) stromal CD8 expression, (C) stromal CD20 expression have excellent correlation on two serial sections of index microarrays stained independently alongside the neoadjuvant cohort. Supplemental Figure 4. Correlation between TIL AQUA scores and clinicopathologic characteristics. Supplemental Figure 5. Determination of cutpoints between low and high expression of TIL biomarkers. Supplemental Figure 6. Sensitivity and specificity for AQUA analysis of CD3, CD8, and CD20 and histopathologic assessment of TILs on H&E slides.
Authors: Jason R. Brown, Hallie Wimberly, Donald R. Lannin, Christian Nixon, David L. Rimm, Veerle Bossuyt
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361883850