Supplementary Figures 1 - 20 from Gene Expression Profile Identifies Tyrosine Kinase c-Met as a Targetable Mediator of Antiangiogenic Therapy Resistance
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 3201K, Supplementary Figure S1. Real time RT PCR analysis of BRGs compared to paired pre-treatment GBMs; Supplementary Figure S2. FISH reveals no change in c-Met amplification after bevacizumab resistance as compared to before; Supplementary Figure S3. Changes in Pearson correlation coefficients to 3 previously described molecular GBM subtypes in BRGs and bevacizumab-na�ve GBMs after recurrence; Supplementary Figure S4. C-Met phosphorylation increases after bevacizumab resistance; Supplementary Figure S5. Increased expression of phosphorylated FAK, a downstream effector of c-Met, in bevacizumab-resistant glioblastomas (BRGs) compared to before treatment; Supplementary Figure S6. Increased expression of phosphorylated STAT3, a downstream effector of c-Met, in bevacizumab-resistant glioblastomas (BRGs) compared to before treatment; Supplementary Figure S7. Hypoxia after bevacizumab resistance versus bevacizumab-na�ve recurrence; Supplementary Figure S8. Endothelial cells do not contribute to increased c-Met expression after bevacizumab resistance; Supplementary Figure S9. Hypoxia increases HGF expression in U87-IgG and U87-BevR cells; Supplementary Figure S10. Xenografts established from BRGs analyzed by immunohistochemistry; Supplementary Figure S11. Immunofluorescent staining of xenografts established from bevacizumab-na�ve GBMs; Supplementary Figure S12. Hematoxylin and Eosin staining of xenografts established from bevacizumab-na�ve GBMs; Supplementary Figure S13. Comparison of xenografts from BRGs versus xenografts from bevacizumab-na�ve GBMs; Supplementary Figure S14. Murine MRI of intracranial BRG-derived xenografts reveals loss of the ability of VEGF blockade to reduce vascular permeability and fractional blood volume; Supplementary Figure S15. Reduced c-Met expression after transducing BRG-derived SF7796 xenograft cells with c-Met shRNA; Supplementary Figure S16. Effects of c-Met knockdown on brain slice invasion in U87-BevR cells; Supplementary Figure S17. Altered morphology in BRG cells after transduction with shRNA targeting c-Met; Supplementary Figure S18. C-Met knockdown alters brain slice invasion in BRG-derived SF7796 cells; Supplementary Figure S19. Hematoxylin and eosin stainings of intracranial SF7796/shControl and SF7796/shCmet1 xenografts; Supplementary Figure S20. Transduction of U87 with retrovirus derived from pBABE-Puro-Tpr-met leads to increased Tpr-met phosphorylation.
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361884175