Figure S5 from Protein Kinase C Epsilon Is a Key Regulator of Mitochondrial Redox Homeostasis in Acute Myeloid Leukemia
Year: 2023
Type: other
Abstract: Supplemental Figure 5. (A) Gating strategy of OCI-AML3 expressing CTRL or PKCε shRNAs and stained with MitoSOX and Annexin V to evaluate mitochondrial superoxide production in viable leukemic cells (Annexin V-negative). (B) THP-1 transduced with CTRL shRNA or PKCε shRNA were stained after 4 days post-transduction with MitoSOX and Annexin V (to exclude dead cells). The left panel is an illustrative histogram plot of a single experiment and the right panel show the MitoSOX MFI of live cells (Annexin V-) expressed as mean {plus minus} SD of three independent experiments. (C) Cells from patient-derived AML sample TUHS-AML-013 transduced with lentiviruses co-expressing GFP and CTRL, PKCε shRNA_1 or SOD2 shRNA_1 shRNA and then analyzed at day 6 post-transduction with MitoSOX levels by flow cytometry. The bar graph shows the MFI of MitoSOX expressed as the mean {plus minus} SD of three technical replicates. (*p{less than or equal to}0.05; ***p{less than or equal to}0.001).
Authors: Daniela Di Marcantonio, Esteban Martinez, Simone Sidoli, Jessica Vadaketh, Margaret Nieborowska-Skorska, Anushk Gupta, Jake M. Meadows, Francesca Ferraro, Elena Masselli, Grant A. Challen, Michael D. Milsom, Claudia Scholl, Stefan Fröhling, Siddharth Balachandran, Tomasz Skorski, Benjamin A. Garcia, Prisco Mirandola, Giuliana Gobbi, Ramiro Garzon, Marco Vitale, Stephen M. Sykes
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361886065