Figure S8 from Protein Kinase C Epsilon Is a Key Regulator of Mitochondrial Redox Homeostasis in Acute Myeloid Leukemia
Year: 2023
Type: other
Abstract: Supplemental Figure 8. (A & B) OCI-AML3 cells transduced with Ctrl or PKCε-expressing retroviruses were analyzed for (A) western blot or (B) cell growth every 2 days by MTS analysis. (C) Bar graph representing the number of colonies formed by CTRL or PKCε-expressing mouse MLL-AF9 leukemia cells seeded at the indicated concentrations in methylcellulose. Data are represented as the mean {plus minus} SD of three technical replicates. (E & F) THP-1 cells were transduced with retroviral vectors that constitutively express PKCε and GFP (PKCε) or just GFP-expressing control (Ctrl) viruses. Four days after transduction, GFP+ cells were isolated by FACS and treated with 80µM of (E) AA or (F) 200µM TTFA. The percentage of Annexin V+ cells was evaluated 24 hours after treatment by flow cytometry. Data are represented as mean {plus minus} SD of three independent experiments. (G & H) U937 cells transduced with lentiviral vectors co-expressing GFP and either CTRL shRNA or PKCε_1 shRNA. Four days after transduction, cells were treated with 100µM of (G) AA or (H) 200µM TTFA and then analyzed 24 hours later for the percentage of Annexin V+ cells by Flow Cytometry analysis. Data are represented as mean {plus minus} SD of three independent experiments. (*p{less than or equal to} 0.05; **p{less than or equal to}0.01).
Authors: Daniela Di Marcantonio, Esteban Martinez, Simone Sidoli, Jessica Vadaketh, Margaret Nieborowska-Skorska, Anushk Gupta, Jake M. Meadows, Francesca Ferraro, Elena Masselli, Grant A. Challen, Michael D. Milsom, Claudia Scholl, Stefan Fröhling, Siddharth Balachandran, Tomasz Skorski, Benjamin A. Garcia, Prisco Mirandola, Giuliana Gobbi, Ramiro Garzon, Marco Vitale, Stephen M. Sykes
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361886087