FigureS7 from Protein Kinase C Epsilon Is a Key Regulator of Mitochondrial Redox Homeostasis in Acute Myeloid Leukemia
Year: 2023
Type: other
Abstract: Supplemental Figure 7. (A-D) OCI-AML3 cells were transduced with lentiviruses expressing CTRL shRNA or SOD2 shRNA and analyzed for: (A) Western blot analysis 4 days after transduction with the indicated antibodies. (B) MitoSOX and Annexin V staining by flow cytometry, 4 days after transduction. The bar graph shows the MitoSOX median fluorescence intensity (MFI) of live (Annexin V-) cells and data is expressed as mean {plus minus} SD of three independent experiments. (C) Cell growth every 2 days by MTS analysis. Data represents the mean {plus minus} SD of three independent replicates. (D) Annexin V staining 5 days after transduction. Data represents the mean {plus minus} SD of three independent replicates. (E) Bar graph representing the number of colonies formed by mouse MLL-AF9 leukemia cells transduced with CTRL shRNA, Sod2 shRNA_1 and Sod2 shRNA_2 in methylcellulose culture. Data is expressed as mean {plus minus} SD of three independent replicates. (*p{less than or equal to}0.05, **p{less than or equal to}0.01; ****p<0.0001). (F) Heatmap analysis displaying the expression of redox-regulatory proteins that were differentially expressed between CTRL shRNA, PKCε shRNA_1 or PKCε shRNA_2 transduced OCI-AML3 cells (4 days after transduction). The proteins differences displayed were not statistically significant.
Authors: Daniela Di Marcantonio, Esteban Martinez, Simone Sidoli, Jessica Vadaketh, Margaret Nieborowska-Skorska, Anushk Gupta, Jake M. Meadows, Francesca Ferraro, Elena Masselli, Grant A. Challen, Michael D. Milsom, Claudia Scholl, Stefan Fröhling, Siddharth Balachandran, Tomasz Skorski, Benjamin A. Garcia, Prisco Mirandola, Giuliana Gobbi, Ramiro Garzon, Marco Vitale, Stephen M. Sykes
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361886173