Supplementary Data from Numb−/low Enriches a Castration-Resistant Prostate Cancer Cell Subpopulation Associated with Enhanced Notch and Hedgehog Signaling
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Mehods, Materials, figures and tables Figure S1. Immunofluorescent staining of Numb in PCa tissues. Figure S2. Classical Wnt signaling was not affected by Numb in PCa cells. Figure S3. The mRNA levels of Hes1 and GLI1 negatively correlated with Numb in PCa tissues. Figure S4. Identification of Numb proximal promoter. Figure S5. Notch signaling exerted no significant effect on the transcription of Numb gene. Figure S6. Effects of the treatment of DAPT, cyclopamine, or a combination of DAPT and cyclopamine on the GFP status of Numb-promoter-GFP reporter lentivirus transfected C4-2B cells. Figure S7. Treatment with a combination of DAPT and cyclopamine resulted in a shift of Numb-/low to Numbhigh cells. Figure S8. The percentage of cells with nuclear localized AR protein was significantly lower in Numb-/low PCa cells than Numbhigh cells. Figure S9. Blockage of Notch and Hedgehog signaling by DAPT and cyclopamine significantly enhanced the apoptosis of Numb-/low cells induced by flutamide-mediated androgen deprivation. Supplementary Table 1. Detailed clinical information of human prostate cancer patients in this study Supplementary Table 2. Primer sequences for qRT-PCR Supplementary Table 3. Antibodies used in Apoptosis Assay, Immunoblotting and Immunofluorescence experiments. Supplementary Table 4. shPTCH1 and shNumb sequences. Supplementary Table 5. Information of plasmids components of RBP-Jκ, GLI and TCF/LEF1 luciferase reporter Supplementary Table 6. Information of response elements of RBP-Jκ, GLI and TCF/LEF1 luciferase reporter
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361886802