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Figure S6 from PDK1 Mediates NOTCH1-Mutated Head and Neck Squamous Carcinoma Vulnerability to Therapeutic PI3K/mTOR Inhibition

Year: 2023
Type: other
Abstract: Figure S6. Effects of PI3K/mTOR pathway inhibition in NOTCH1WT HNSCC with and without NOTCH1 knock out (KO). Two NOTCH1WT HNSCC cell lines (FaDU and MDA686LN), parental cell lines, and NOTCH1 KO cell lines were treated with GSK2126458 (GSK212). A and B. The CellTiter-Glo assay was used to assess the viability of cells treated for 72 hours with the indicated concentrations of GSK212. C and D. Annexin V/propidium iodide staining was used to assess the apoptosis of cells treated with 50 nM GSK212 for 48 hours. Values are the means {plus minus} standard deviations of three independent experiments; n.s., not significant, unpaired two-tailed Student t-test. (E) Western blot analysis of apoptotic markers was performed after treatment with the indicated concentrations of GSK212 for 48 hours.
DOI: 10.1158/1078-0432.22469300
Authors: Vaishnavi Sambandam, Mitchell J. Frederick, Li Shen, Pan Tong, Xiayu Rao, Shaohua Peng, Ratnakar Singh, Tuhina Mazumdar, Chenfei Huang, Qiuli Li, Curtis R. Pickering, Jeffery N. Myers, Jing Wang, Faye M. Johnson
Language: English
Open Access status: gold
OpenAlex ID: W4361887115
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