Figure S9 from PDK1 Mediates NOTCH1-Mutated Head and Neck Squamous Carcinoma Vulnerability to Therapeutic PI3K/mTOR Inhibition
Year: 2023
Type: other
Abstract: Figure S9. PDK1 inhibition and AKT inhibition act synergistically against NOTCH1MUT head and neck squamous cell carcinoma (HNSCC). A. Two NOTCH1WT lines and four NOTCH1MUT lines were treated with 100 nM BAY806946 or 1 µM PQR309 for 24 hours and then subjected to Western blot analysis with the indicated antibodies. B. Parental and PDK1-overexpressing NOTCH1MUT cells were treated with 50 nM GSK212 for 24 hours and then subjected to Western blot analysis with the indicated antibodies. C. Three NOTCH1MUT HNSCC lines were treated with increasing concentrations (0-10 µM, 2-fold dilution) of MK2206 and/or GSK2334470 for 72 hours, and cell viability was assessed using the CellTiter-Glo assay. D. The combination index (CI) for the fractions affected (Fa) for each combination was calculated with the Chou Talalay method using Calcusyn (Biosoft, Cambridge, UK).
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361887216