Supplementary Methods, Figures 1 - 9, Tables 1 - 2 from Sirtuin-1 Regulates Acinar-to-Ductal Metaplasia and Supports Cancer Cell Viability in Pancreatic Cancer
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 790K, S Table 1. Primary and secondary antibodies used in immunostaining and Western Blotting experiments S Table 2. Antibodies used for immunoprecipitation experiments S Table 3. Primers used in real time RT-PCR and PCR of the ChIP experiments S Figure 1: The expression pattern of Sirt1 and beta-catenin changes in Caerulein induced pancreatitis. S Figure 2: The expression pattern of Sirt1 and beta-catenin, but not Dbc1, changes in pancreatic duct ligation. S Figure 3: In an in vitro ADM model, Sirt1 undergoes transient changes in subcellular localisation without changes in expression levels. S Figure 4: Deletion of functional Sirt1 in Pdx1-Cre;Sirt1ex4lox/lox pancreas impacts on acinar cell differentiation during ADM. S Figure 5: Acinar cell differentiation is not altered during ADM in Sirt1 transgenic (SirtTg) pancreas. S Figure 6: Nicotinamide represses ADM of human cells in culture. S Figure 7: Sirt1 co-localizes and interacts with beta-Catenin in acinar cells. S Figure 8: Nicotinamide increases beta-catenin/Wnt signaling in ADM cultures. S Figure 9: Dbc1 expression is decreased in PDAC but not in chronic pancreatitis.
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361895615