Supplemental Figures from TWIST1-WDR5-Hottip Regulates Hoxa9 Chromatin to Facilitate Prostate Cancer Metastasis
Year: 2023
Type: supplementary-materials
Abstract: This document contains supplemental details such as antibodies and primers, list of genes overrepresented in TWIST1 and TWIST1 mutants, IHC staining for TWIST1 in mouse prostate development, IHC staining for TWIST1 and HOXA9 in various mouse models of prostate cancer, correlation of TWIST1 and HOXA9 alteration with poor survival in human prostate cancer patients, IHC for TWIST1 and HOXA9 on primary tumors and bone metastasis from prostate cancer patients, IHC for HOXA9 on cell pellets expressing HOXA9 or control and on adult mouse prostate, data showing sufficiency of HOXA9 for some pro-metastatic behaviour in prostate cancer cells, data showing requirement of WDR5 and HOTTIP for TWIST1-dependent pro-metastatic behavior, ChIP data showing binding of TWIST1 and HOXA9 at the HOXA9 promoter, data showing effect of drugs that can inhibit HOXA9 on prostate cancer cell viability and pro metastatic behaviour.
Authors: Reem Malek, Rajendra P. Gajula, Russell D. Williams, Belinda Nghiem, Brian W. Simons, Katriana Nugent, Hailun Wang, Kekoa Taparra, Ghali Lemtiri-Chlieh, Arum R. Yoon, Lawrence True, Steven S. An, Theodore L. DeWeese, Ashley E. Ross, Edward M. Schaeffer, Kenneth J. Pienta, Paula J. Hurley, Colm Morrissey, Phuoc T. Tran
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361897447