Fig. S6 from Metastatic Conditioning of Myeloid Cells at a Subcutaneous Synthetic Niche Reflects Disease Progression and Predicts Therapeutic Outcomes
Year: 2023
Type: other
Abstract: Statistical plots to support heatmaps in main text and supplementary materials. (a) Box plots for gene panel heatmap in Fig. 1f show the median, 25th-75th percentiles and most extreme data points that were not considered outliers (outliers are visually indicated by red +). TB cohort expression is centered relative to the time-matched TFC cohort median. Scale depicts the log2-transformed fold change. A two-way MANOVA showed a significant interaction effect between condition and time (Pillai's Trace=1.276, F(20,28)=2.465, p=0.014). Post hoc univariate ANOVA showed significant differences within the diseased cohort over time (indicated by #, df=(2,22), p<0.05). Simple effects analysis showed significant differences between diseased and time-matched TFC (indicated by *, Šidák adjusted p<0.05 adjusted). (b) Tumor growth curve for 4T1 mice starting at Day 7, with a power regression line, error bars are standard deviation. (c) Box plots of gene expression to support the data in the C57BL/6-Lu.2 heatmap in Fig. 2b. * indicates significance (Dunn-Šidák corrected to p<0.005) from independent two-tailed t-tests comparing TB to TFC (n=5 per condition). (d) Box plots of gene expression for blood data to support the heatmap found in Fig. 1. Tumor-bearing Implant Free Controls (IFCs) are shown to illustrate that the implants do not alter the gene expression of blood leukocytes. A two-way MANOVA showed a significant interaction effect between condition and time (Pillai's Trace=2.527, F(36,40)=1.907, p=0.024). Post hoc univariate and simple effects analysis showed significant differences within the diseased cohort over time as indicated as # (df=(2,15), p<0.001) and * indicates significant differences compared to time matched controls (Šidák adjusted p<0.05 adjusted). (e) Gene expression data from the lung tissue heatmap in Fig. 2a. * indicates significance (Dunn-Šidák corrected to p<0.005) from independent two-tailed t-tests comparing TB to TFC (n=4 per condition). For full statistical values see Data File 4 for exact F and p values.
Language: English
Topic: Cancer Cells and Metastasis
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361898616