Supplementary Figures 1 - 6, Tables 1 - 2, Methods from The Ability of Plasma Cotinine to Predict Nicotine and Carcinogen Exposure is Altered by Differences in CYP2A6: the Influence of Genetics, Race, and Sex
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 215K, Supplementary Table 1: Descriptive participant characteristics. Supplementary Table 2: Regression analyses of the predictive ability of cotinine and CYP2A6 genotype (or sex) on tobacco and tobacco-derived carcinogen exposure (Study 2). Supplementary Figure 1. Within the male participants (Study 1): A. CYP2A6 RM (n=10) metabolized a significantly lower amount nicotine to cotinine compared to CYP2A6 NM (n=44). B. CYP2A6 RM (n=10) had lower cotinine clearance (CLCOT) compared to CYP2A6 NM (n=44). C. The fNICCOT/CLCOT ratio was higher in CYP2A6 RM compared to CYP2A6 NM. Within the female participants (Study 1): D. CYP2A6 RM metabolized a significantly lower amount of nicotine to cotinine compared to CYP2A6 NM. E. CYP2A6 RM had lower cotinine clearance (CLCOT) compared to CYP2A6 NM. F. The fNICCOT/CLCOT ratio was higher in CYP2A6 RM compared to CYP2A6 NM. With all participants (Study 1): G. Individuals with slower NMR metabolized a significant lower amount of nicotine to cotinine compared to the individuals with faster NMR. H. Individuals with slower NMR had lower cotinine clearance (CLCOT) compared to the individuals with faster NMR. I. fNICCOT/CLCOT was higher among individuals in the slower NMR stratum compared to the individuals in the faster NMR stratum. Data presented as mean � 95% confidence interval. Supplementary Figure 2: Cotinine�s ability to predict tobacco exposure was different between NMR strata (Study 2). Supplementary Figure 3. TNE�s ability to predict tobacco specific nitrosamines exposure is independent of CYP2A6 genotype (Study 2).Supplementary Figure 4. Cotinine�s ability to predict polycyclic aromatic hydrocarbon exposure (as indicated by 1-hydroxyfluorene) was different between CYP2A6 genotypes (Study 2). Supplementary Figure 5. Among the CYP2A6 normal metabolizers (Study 1, n=148), males, who have reduced CYP2A6 activity compared to females, had a greater ratio of cotinine formation over removal.Supplementary Figure 6. A nicotine patch study: abstinent individuals with slower NMR have higher steady state plasma cotinine compared to individuals with faster NMR after receiving the same 21 mg nicotine patch.
Language: English
Subfield: Pharmacology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361901696