Supplementary Tables 1 - 2 from Activity of the Hypoxia-Activated Prodrug, TH-302, in Preclinical Human Acute Myeloid Leukemia Models
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 66K, Supplemental Table 1. Cell cycle analysis of AML cells treated with TH-302. AML cells were treated with vehicle (PBS) or TH- 302 at the indicated concentrations for 24 hours under hypoxic (1% O2) or normoxic (21% O2) conditions in triplicate wells. Cells were stained with propidium iodide staining and analyzed by flow cytometry. Mean plus-minus SEM percentages of cells in each cell cycle phase are shown. Similar results were obtained in three independent experiments. Supplemental Table 2. TH-302 induces dose and hypoxia-dependent apoptosis in representative human AML cell lines. Shown are the mean plus-minus SEM percentages of total apoptotic and dead cells as determined by flow cytometry for 7-AAD/annexin-V expression in a panel of seven human AML cell lines. Known molecular and cytogenetic characteristics of each cell line is provided. Cells were incubated with vehicle or TH-302 at doses ranging from 0.1-10 μM under normoxia (21% O2) or hypoxia (1% O2) for 72 hours. Cells were plated in multiple wells per condition with summarized results of at least two independent experiments provided. Results of student t test analysis comparing results under normoxia vs. hypoxia are provided.
Authors: Scott Portwood, Deepika Lal, Yung-Chun Hsu, Rodrigo Vargas, Megan K. Johnson, Meir Wetzler, Charles P. Hart, Eunice S. Wang
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361882067