Supplementary Figures 1 - 8 from Transient Activation of Hedgehog Pathway Rescued Irradiation-Induced Hyposalivation by Preserving Salivary Stem/Progenitor Cells and Parasympathetic Innervation
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 2605K, Fig. S1. X-gal staining of SMG sections from Ptch1-lacZ mice non-treated (NT), 7 days after ligation of main excretory ducts or IR. Fig. S2. 7 days of Dox treatment did not rescue IR-induced hyposalivation in female KrtrtTA/ tetO-Shh mice and male wild type mice. (A) Relative saliva flow rate. (B) Relative expression of acinar markers Aqp5 and Chrm3 determined by qRT-PCR. Fig. S3. Transient Hh activation has no long-term effect on percentage of putative salivary stem/progenitor cells. Fig. S4. Effects of transient Hh activation on proliferation and IR-induced apoptosis of SMG cells. Fig. S5. Co-expression of makers for salivary stem/progenitor cells with Hh target genes. Fig. S6. Activation of Hh pathway in female mouse SMGs by SAG. Fig. S7. Morphology of cultured human salivary epithelial cells. Fig. S8. Effects of retrograde Shh gene transfer into SMGs on expression of Hh target genes and SCC VII tumor growth.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361907915