Year: 2023
Type: other
Abstract: PDF file 162K, Figure S3. (A) MTT assay results showing the cytotoxicity of KX-01 at various concentrations in clear ovarian carcinoma cells. Cell viability was calculated as the number of cells surviving relative to the number of cells surviving in the control group. IC50 indicates median inhibitory rate. (B) Western blot results showing that the caspase pathway was activated to generate cleaved PARP. Total extracts from RMUG-S and RMUG-L cells treated with 100nM KX-01 for the indicated times were analyzed using the anti-PARP antibody, which detected endogenous levels of full-size (116-kDa) and large-fragment (89-kDa) PARP produced by caspase cleavage. {beta}-actin was used as the loading control. (C, D) Cell migration in RMUG-S and L cells treated with 100 nM KX-01 was analyzed by using a Boyden chamber assay. Data are shown as mean {plus-minus} standard deviation (error bars)
Authors: Tao Liu, Wei Hu, Heather J. Dalton, Hyun Jin Choi, Jie Huang, Yu Kang, Sunila Pradeep, Takahito Miyake, Jian H. Song, Yunfei Wen, Chunhua Lu, Chad V. Pecot, Justin Bottsford-Miller, Behrouz Zand, Nicholas B. Jennings, Cristina Ivan, Gary E. Gallick, Keith A. Baggerly, David G. Hangauer, Robert L. Coleman, Michael Frumovitz, Anil K. Sood
Language: English
Subfield: Reproductive Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361908070