Supplementary Figure 3 from PLK1 Inhibitors Synergistically Potentiate HDAC Inhibitor Lethality in Imatinib Mesylate–Sensitive or –Resistant BCR/ABL+ Leukemia Cells In Vitro and In Vivo
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 82K, Knock-down of histone 1.2 protects K562 cells from the lethal effects of combined BI2536 and vorinostat treatment. (A) K562 cells were transiently transfected with scramble sequence or shH1.2 as described in Methods and treated with BI2536 (4.0 nM) and vorinostat (1.25 �M) for 48 hr. Cell death was monitored by 7AAD staining and flow cytometry. Inset: Western blots showing expression of histone 1.2 expression in empty vector control and knockdown cells. (B) K562 cells were transiently transfected with scramble sequence or shH1.2 as described in Methods and treated with BI and vorinostat as (A) above for 24 hr, after which expression of the indicated proteins was monitored by western blotting.
Authors: Girija Dasmahapatra, Hiral Patel, Tri Nguyen, Elisa Attkisson, Steven Grant
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361911348