Supplementary Figure 4 from PLK1 Inhibitors Synergistically Potentiate HDAC Inhibitor Lethality in Imatinib Mesylate–Sensitive or –Resistant BCR/ABL+ Leukemia Cells In Vitro and In Vivo
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 104K, Co-treatment with BI2536 or PLK-1 knockdown by shRNA and vorinostat arrests cells in G2M phase. (A) K562 cells were treated with 5.0 nM BI2536 � 1.5 �M vorinostat. (B-C) K562 cells transiently transfected with scramble sequence or shPLK-1 and treated with vorinostat (1.5 �M) for 24 hr. Following drug exposure, cells were collected, fixed in ice cold methanol at a ratio of 1 mL PBS to 4 mL ice cold ethanol, and analyzed by flow cytometry using Modfit software as described in Methods. Duplicate experiments yielded equivalent results.
Authors: Girija Dasmahapatra, Hiral Patel, Tri Nguyen, Elisa Attkisson, Steven Grant
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361911399