Supplementary Figure S1 from Analytic Validation of RNA In Situ Hybridization (RISH) for AR and AR-V7 Expression in Human Prostate Cancer
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figure S1: AR-E1 and AR-CE3 RISH assays are specific. (A) AR-E1 is undetectable in AR-negative, untransfected M12 cells. Transfection with AR-FL construct is detectable by AR_E1, but not by AR_CE3 RISH as expected in M12 cells. (B) Untransfected HeLa cells have low to undetectable levels of AR-E1. Transfection with AR-E1-containing construct leads to highly detectable AR_E1 by RISH. (C) RISH with probes detected human AR_E1 sequence is negative in murine prostate. As a positive control for RNA quality, RISH for c-myc is positive in these transgenic mice which express the c-myc transgene.
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361920539