Supplementary Figure S4 from Analytic Validation of RNA In Situ Hybridization (RISH) for AR and AR-V7 Expression in Human Prostate Cancer
Year: 2023
Type: supplementary-materials
Abstract: AR-E1 and AR-CE3 RISH assay digital image quantification shows minimal inter-observer variability. To examine inter-observer variability in scoring, a second, independent operator (Observer 2) circled tumor areas and performed the scoring for AR-E1 and AR-CE3 in Frida across a subset of the autopsy samples and compared with the original values created by Observer 1 for Supplementary Figure S3C. In one experiment, the second operator made their own thresholds for brown and blue intensity scoring and in another experiment, the second operator used the intensity thresholds set by the first operator. In both cases there was negligible variability between operators, with highly correlated values across all specimens.
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361920547