Supplementary Data from Circulating Tumor Cells from Different Vascular Sites Exhibit Spatial Heterogeneity in Epithelial and Mesenchymal Composition and Distinct Clinical Significance in Hepatocellular Carcinoma
Year: 2023
Type: supplementary-materials
Abstract: Figure S1. Dynamic change of the clustering status of circulating tumor cells (CTCs) along their circulating route. Figure S2. Variation of circulating tumor cell (CTC) clustering status between primary hepatocellular carcinoma (HCC) efferent and afferent micovessels. Example of circulating tumor microemboli (CTM) detected in microscopic hepatic vein (mHV, efferent vessel) and singular CTCs in microscopic hepatic artery (mHA, afferent vessel) stained for epithelial (E) and mesenchymal (M) markers. E-cad = E-cadherin, red; CK = cytokeratin, brown; vit = vimentin, brown. Scale bar: original magnification, 100μm; inserts, 25 μm. Figure S3. Determination of epithelial-mesenchymal transition (EMT)-related marker expression on hepatocellular carcinoma (HCC) cell lines. Figure S4. Circulating tumor cells (CTCs) isolated using the CELLSEARCH® system were verified by confocal microscopy. Figure S5. Scatter plots showing the comparison of the cell counts of each EMT-related CTC phenotype from five vascular sites, including HV, PA, PV, IHIVC, and PoV (*P<0.05, **P<0.01, n.s. = not significant, Wilcoxon signed-rank test). Figure S6. The proportional distribution of EMT-related CTC phenotypes based on E and M staining at five key vascular sites. Figure S7. Receiver operating characteristic (ROC) curve analysis for range of circulating tumor cell (CTC) cutoff values. Table S1. Anatomic distribution of CTCs and CTMs Table S2 Pairwise comparison of CTC and CTM detected at different gross vascular sites Table S3. The correlation between primary tumor EMT status and CTC EMT status detected in gross and microscopic hepatic vein
Authors: Yun-Fan Sun, Wei Guo, Yang Xu, Yin-Hong Shi, Zi-Jun Gong, Yuan Ji, Min Du, Xin Zhang, Bo Hu, Ao Huang, George G. Chen, Paul B.S. Lai, Ya Cao, Shuang-Jian Qiu, Jian Zhou, Xin-Rong Yang, Jia Fan
Language: English
Topic: Cancer Cells and Metastasis
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361922582