Figure S3 from Protein Kinase C Epsilon Is a Key Regulator of Mitochondrial Redox Homeostasis in Acute Myeloid Leukemia
Year: 2023
Type: other
Abstract: Supplemental Figure 3. (A) Schematic representation of the experimental design for analysis of patient derived (PD) - AML samples. (B) Ex vivo growth curve analysis of patient-derived AML sample TUHS-AML-013 transduced with lentiviruses co-expressing GFP and CTRL or PKCε shRNA_1 and then analyzed by flow cytometry for the GFP+, CD45+, PI- cells every 3 days following transduction as discussed in the legend of Figure 2 (*p{less than or equal to}0.05). (C) NOMO1 and (D) THP-1 cells were transduced with CTRL shRNA or PKCε shRNAs and stained 5 days later with CellROX. The left panel is an illustrative histogram plot of a single experiment and the right panel shows the Median Fluorescence Intensity (MFI) of CellROX (**p{less than or equal to}0.01; ****p<0.0001). Data are represented as the mean {plus minus} SD of three technical replicates.
Authors: Daniela Di Marcantonio, Esteban Martinez, Simone Sidoli, Jessica Vadaketh, Margaret Nieborowska-Skorska, Anushk Gupta, Jake M. Meadows, Francesca Ferraro, Elena Masselli, Grant A. Challen, Michael D. Milsom, Claudia Scholl, Stefan Fröhling, Siddharth Balachandran, Tomasz Skorski, Benjamin A. Garcia, Prisco Mirandola, Giuliana Gobbi, Ramiro Garzon, Marco Vitale, Stephen M. Sykes
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361925158