Figure S1 from Protein Kinase C Epsilon Is a Key Regulator of Mitochondrial Redox Homeostasis in Acute Myeloid Leukemia
Year: 2023
Type: other
Abstract: Supplemental figure 1. THP-1, NOMO1, OCI-AML3 and U937 cell lines were transduced with CTRL shRNA or PKCε shRNA_1, or PKCε shRNA_2 and analyzed for (A) PKCε expression by Western Blot analysis 4 days after transduction or (B) cell growth every 2 days by MTS analysis (Day 4, CTRL shRNA vs. PKCε shRNA_1 or _2. ***p{less than or equal to}0.001;****p<0.0001). (C) Five days after transduction, cells were stained with Annexin V and analyzed by flow cytometry. Data are represented as the mean {plus minus} SD of three technical replicates. (***p{less than or equal to}0.001;****p<0.0001). (D) Seven day after transduction, THP-1 and OCI-AML3 were analyzed by flow cytometry for CD11b expression (***p{less than or equal to}0.001;****p<0.0001). (E) Wright - Giemsa staining of THP-1 infected with CTRL shRNA or PKCε shRNA_1 and analyzed 7 days after infection (100X magnification - bar = 100ï�m)
Authors: Daniela Di Marcantonio, Esteban Martinez, Simone Sidoli, Jessica Vadaketh, Margaret Nieborowska-Skorska, Anushk Gupta, Jake M. Meadows, Francesca Ferraro, Elena Masselli, Grant A. Challen, Michael D. Milsom, Claudia Scholl, Stefan Fröhling, Siddharth Balachandran, Tomasz Skorski, Benjamin A. Garcia, Prisco Mirandola, Giuliana Gobbi, Ramiro Garzon, Marco Vitale, Stephen M. Sykes
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361925263