Figure S5 from PDK1 Mediates NOTCH1-Mutated Head and Neck Squamous Carcinoma Vulnerability to Therapeutic PI3K/mTOR Inhibition
Year: 2023
Type: other
Abstract: Figure S5. Effects of PI3K/mTOR signaling inhibition on the survival of NOTCH1MUT and NOTCH1WT head and neck squamous cell carcinoma (HNSCC) cell lines in vitro. A. The Annexin V/propidium iodide assay was used to assess apoptosis in HNSCC cells that had the indicated NOTCH1 and PIK3CA mutations and were treated with 500 nM BAY806946 or 1 µM PQR309. Values are the means {plus minus} standard deviations of three independent experiments. *p<0.05, unpaired two-tailed Student t-test. B. NOTCH1MUT lines (red text) and NOTCH1WT lines (black text) were treated with increasing concentrations of PQR309 and BAY806946 for 3 hours, and then Western blotting was used to assess levels of p-AKT (S473). C. NOTCH1WT lines (black text), NOTCH1MUT lines (red text), and PIK3CAMUT lines (blue text) were treated with 50 nM GSK2126458 (GSK212) and then pulse-labeled with BrDU and counterstained with 7-AAD for flow cytometry.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361932919