Figure S7 from PDK1 Mediates NOTCH1-Mutated Head and Neck Squamous Carcinoma Vulnerability to Therapeutic PI3K/mTOR Inhibition
Year: 2023
Type: other
Abstract: Figure S7. Effects of the combined inhibition of the PI3K/mTOR and NOTCH1 pathways in NOTCH1WT head and neck squamous cell carcinoma (HNSCC). A. Four NOTCH1WT HNSCC cell lines were treated with the indicated concentrations of GSK2126458 (GSK212) and 20 µM N-[N-(3,5-difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester (DAPT) or 20 µM dibenzazepine (DBZ) for 72 hours, and cell viability was assessed with the CellTiter-Glo assay. Representative dose-response curves are shown. B. The combination index (CI) for each fraction affected (Fa) was calculated using the Chou-Talalay method. C. Western blotting for apoptosis markers after treatment with 50 nM GSK212 and 20 µM DAPT for 48 hours. D and E. NOTCH1WT cells were treated with 50 nM GSK212 and 20 µM DBZ or DAPT for 48 hours, and apoptosis was measured using an Annexin V/propidium iodide assay. The percentages of apoptotic cells are expressed as the means {plus minus} standard deviations of three independent experiments. *p<0.001, **p<0.05, two-way ANOVA corrected for multiple comparisons by the Tukey test; ns, not significant.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361933910