Supplementary Figure S2 from Establishment of Patient-Derived Succinate Dehydrogenase–Deficient Gastrointestinal Stromal Tumor Models for Predicting Therapeutic Response
Year: 2023
Type: supplementary-materials
Abstract: Fig. S2. Recapitulation of metabolic characteristics of parental tumors and SDH-deficiency by patient-derived mSDH GIST models. A. Concentration of succinate in wildtype (WT) (n = 2), mSDHA (n = 1), mSDHB (n = 3), and mSDHC (n = 1) patient plasma. B. Ratio of succinate to fumarate in WT (n = 2), mSDHA (n = 1), mSDHB (n = 3), and mSDHC (n = 1) patient plasma. C. Percent labeling of M3 pyruvate from [U-13C5] glucose in mSDH GIST models grown for 48 h. D. Percent labeling of M3 lactate from [U-13C5] glucose in mSDH GIST models grown for 48 h. E. Per cell abundance of 2-hydroxyglutarate (2-HG) relative to WT in mSDH GIST models grown for 48 h. One-way ANOVA was performed for statistical analysis with *, P<0.05; ***, P<0.001; ****, P<0.0001. F. mSDH GIST models were treated for 72 h with either DMSO or a 10-point dose range of 2-DG and cell viability was measured using the CellTiter-Glo viability assay. G. mSDH GIST models were treated for 72 h with either DMSO or a 10-point dose range of 6-AN and cell killing was measured using the CellTiter-Glo viability assay.
Authors: Mayra Yebra, Shruti Bhargava, Avi Kumar, Adam M. Burgoyne, Chih-Min Tang, Hyunho Yoon, Sudeep Banerjee, Joseph Aguilera, Thekla Cordes, Vipul Sheth, Sangkyu Noh, Rowan Ustoy, Sam Li, Sunil J. Advani, Christopher L. Corless, Michael C. Heinrich, Razelle Kurzrock, Scott M. Lippman, Paul T. Fanta, Olivier Harismendy, Christian Metallo, Jason K. Sicklick
Language: English
Subfield: Gastroenterology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361961976