Supplementary Fig.1 from Interleukin-7 Mediates Selective Expansion of Tumor-redirected Cytotoxic T Lymphocytes (CTLs) without Enhancement of Regulatory T-cell Inhibition
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Fig.1 PDF file 106K, A. Gating strategy to evaluate the transduction efficiency for the CAR-GD2. The gate was drawn based on the physical parameters and the expression of the CAR-GD2 evaluated on gated cells. B. Gating strategy to evaluate the transduction efficiency for IL-7Ra. The gate was drawn based on the physical parameters and the expression of IL-7Ra evaluated on gated cells. C. Gating strategy to evaluate the phosphorylation of STAT5. The gate was drawn based on the physical parameters and the phosphorylation of STAT5 evaluated on gated cells. D. Gating strategy to assess CSFE dilution. After gating on lymphocyte, the dilution of the CFSE was assessed on CD8+ cells. Two independent experiments are shown. E. Gating strategy to assess the elimination of tumor cells in co-culture experiments. The gate was drawn based on the physical parameters. Lymphocytes were identified based on CD3 expression and tumor cells by GFP expression on gated cells
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361968777