Supplementary Figures S1-S9 from Astrocyte Elevated Gene-1 Interacts with Akt Isoform 2 to Control Glioma Growth, Survival, and Pathogenesis
Year: 2023
Type: supplementary-materials
Abstract: Supplementary Figures S1-S9. Co-Immunoprecipitation analysis of endogenous AEG-1 following Akt3 OE in glioma cell lysates. (S1); TCGA data analysis: Kaplan-Meir survival of GBM patients (n = 380) from TCGA datasets (S2); Analysis of glioma TMA Immunohistochemical staining scores (S3); OE of PH domains of Akt1 and Akt2 and Akt isoform specific knockdown with RNAi (S4); Cell proliferation assays. U87 cells transfected with AEG- 1, Akt1-PH and Akt2-PH in different combinations as indicated in the figure (S5). AEG-1 enhances the half-life of phospho-Akt S473 (S6); U87 parental cells and cells stably expressing AEG-1 transfected with/without Akt2-PH. Cells were treated with 20 μM CAPE (Caffeic Acid Phenethylester) and collected for immunoblotting (S7); Protein sequence alignment of human Akt isoforms (S8); Details of TCGA analysis (S table 1); Details of brain tumor TMA analysis (S table 2); AEG-1 does not regulate p-Akt1-S473 (S9).
Language: English
Subfield: Pathology and Forensic Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361969350