Supplementary Figure 5 from Chk2 Phosphorylation of Survivin-ΔEx3 Contributes to a DNA Damage–Sensing Checkpoint in Cancer
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 59K, Expression of Aurora B during DNA damage. HCT116 cells on glass coverslips were transfected with the indicated cDNAs, treated with etoposide for 16 h and fixed in 1% paraformaldehyde for 15 min and 70% ethanol for 30 min. After permeabilization in 0.2% Triton X-100 in 1% BSA in PBS, pH 7.4, for 10 min, cells were incubated with primary antibody to Aurora B (1:500) followed by secondary antibody (Alexa Fluor� 594) and analyzed by fluorescence microscopy. DNA was stained with DAPI.
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361969392