Supplementary Data from Intratumoral Adaptive Immunosuppression and Type 17 Immunity in Mismatch Repair Proficient Colorectal Tumors
Year: 2023
Type: supplementary-materials
Abstract: supplementary methods, tables and figures Supplementary Table S1. Demographics of patients from clinical trial NCT01876511 Supplementary Table S2. Demographics of untreated CRC patients Supplementary Table S3. Target genes and specific primer/probes gene expression assay used for Taqman PCR Supplementary Table S4. Summary statistics of immune related gene expression between responder (R) and non-responder (NR) mCRC treated with pembrolizumab, sorted by Wilcoxon-Mann Whitney p-value Supplementary Table S5. Significant p values (p<0.05) of gene expression between newly defined R-type and NR-type MMRp specimens. Supplementary Table S6. Summary statistics for paired differences of gene expression between cell-sorted CD8+ PD1hi and CD8+ PD1lo TILs of resected primary CRC. Gene expression in PD1hi and PD1lo were relative to expression in PD1neg CD8+ TILs (��Ct). Supplementary Table S7. Th1 and mixed Th1/Th17 immune signatures in MSS samples extracted from TCGA database Supplementary Figure S1. Model of TiME in primary colon tumor of mCRC patients according to their clinical response to pembrolizumab. Supplementary Figure S2. IFN�-producing PD1hi CD8+ T cells are not detected in the distal normal colon tissue. Supplementary Figure S3. Cell-sorted PD1hiCD8+ TIL in CRC are exhausted T cells. Supplementary figure S4. MSI high MMRd CRC 3784 TIL includes only few IFN�+PD1hi CD4+ or CD8+ T cells. Supplementary Figure S5. Gene expression analysis in conventional MMRp, type 1 immunoreactive MMRp and type 17 immunoreactive MMRp colon tumors. Supplementary Figure S6. IHC staining on type 17 immunoreactive MMRp CRC. Supplementary Figure S7. IHC staining on conventional MMRp CRC. Supplementary Figure S8. Correlation between the PD-L1 expression in IF of primary colon tumors and the proportion of Th1 and Th17 in TILs. Supplementary Figure S9. CD3 and CD8 cells quantification by IHC and tumor mutation numbers in untreated resected primary CRC lesion. Supplementary Figure S10. Detection of Type 1 and Type 17 immunoreactive MMRp CRC samples in TCGA database.
Authors: Nicolas J. Llosa, Brandon Luber, Ada J. Tam, Kellie N. Smith, Nicholas Siegel, Anas H. Awan, Hongni Fan, Teniola Oke, JiaJia Zhang, Jada Domingue, Elizabeth L. Engle, Charles A. Roberts, Bjarne R. Bartlett, Laveet K. Aulakh, Elizabeth D. Thompson, Janis M. Taube, Jennifer N. Durham, Cynthia L. Sears, Dung T. Le, Luis A. Diaz, Drew M. Pardoll, Hao Wang, Robert A. Anders, Franck Housseau
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361969747