Supplementary Figure 2 from Image Analysis–based Assessment of PD-L1 and Tumor-Associated Immune Cells Density Supports Distinct Intratumoral Microenvironment Groups in Non–small Cell Lung Carcinoma Patients
Year: 2023
Type: supplementary-materials
Abstract: Microphotographs of representative examples of the algorithms used to analyze TAICs density. A) Representative positive CD3 staining (brown) in an ADC showing five random square areas (1 mm2 each; green outlined boxes) in both intratumoral (right) and peritumoral (left) compartments divided by a red line. B) High power images of CD3 IHC expression (left) and image analysis quantification using the nuclear algorithm (right). C) High power images of CD4 IHC expression (left) and image analysis quantification using the nuclear algorithm (right); the algorithm excluded a macrophage cell from analysis as indicated by the arrows. D) higher power images of granzyme B IHC expression (left) and image analysis quantification using the nuclear algorithm (right); E) High power images of FOXP3 IHC expression (left) and image analysis quantification using the nuclear algorithm (right). F) High power images of CD68 (macrophages) IHC expression (left) and image analysis quantification using the cytoplasmic algorithm (right).
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4361970175