Supplementary Figure 2 from Gliomas Promote Immunosuppression through Induction of B7-H1 Expression in Tumor-Associated Macrophages
Year: 2023
Type: supplementary-materials
Abstract: PDF file - 42K, Figure S2. Glioma-derived soluble factors can further induce B7-H1 expression in non-autologous GBM patient peripheral monocytes, but not in autologous patient monocytes. Quantification of mean fluorescence intensity for B7-H1 staining measured by flow cytometry is shown for monocytes stimulated with IL-10 (10 ng/ml) or glioma-conditioned media (SF1U-CM). In monocytes from healthy control patients, B7-H1 intensity was significantly increased by IL-10, and further increased by conditioned media (* p<0.05). Similar results were observed with stimulation of non-autologous monocytes from a GBM patient (* p<0.05, **p<0.01). Monocytes from an autologous GBM patient, SF1U, had high initial B7-H1 expression and could not be further increased with stimulation. Columns represent mean fluorescence intensity plus-minus SEM for each sample repeated in triplicate.
Authors: Orin Bloch, Courtney A. Crane, Rajwant Kaur, Michael Safaee, Martin J. Rutkowski, Andrew T. Parsa
Language: English
Topic: Immune cells in cancer
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361971325